Titlebook: Skin Stem Cells; Methods and Protocol Kursad Turksen Book 2024Latest edition The Editor(s) (if applicable) and The Author(s), under exclusi

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Characterization of the Newborn Epidermis and Adult Hair Follicles Using Whole-Mount Immunofluoresc undergoes rapid cell division and differentiation to form multiple stratified layers of keratinocytes. Concurrently the epidermis also gives rise to hair follicles that invaginate into the dermis. In adult skin, the hair follicle undergoes cyclic regeneration fueled by hair follicle stem cells loca

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Efficient Generation of CRISPR/Cas9-Mediated Knockout Human Primary Keratinocytes by Electroporatiobasic biological and dermatological research questions, including those related to skin diseases and the investigation of treatment options. When modified with the CRISPR/Cas9 gene editing approach and cultivated in organotypic 3D epidermal equivalents (EEs), these human cells have the potential to

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Application of CRISPR/Cas9 Pooled Screening for a Non-immediate Readout Model,e such a goal. Screens can be conducted in either pooled or arrayed formats. Although arrayed screenings provide a better and cheaper alternative in small scale, the larger-scale screenings are conducted in pooled manner. With its adaptability to various models and conditions, CRISPR/Cas9 technology

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Analysis of Proliferation, Apoptosis, and Motility in Mouse Embryonic Melanocytic Precursor Cells,ific manner. Isolation of melanocytic lineage cell populations that are >98% pure is accomplished through the use of GFP-based fluorescence activated cell sorting. We also provide a method to culture the purified melanoblasts and to analyze their proliferation, apoptosis, and motility properties.

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Incorporating NK Cells in a Three-Dimensional Organotypic Culture System for Human Skin Stem Cells: exposed to environmental influences. Recent studies have shown that NK cell incorporation into three-dimensional (3D) organotypic culture systems for human skin stem cells provides a physiologically relevant environment to study the interactions between immune cells and skin cells, making it a powe

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