Titlebook: Skeletal Muscle Regeneration in the Mouse; Methods and Protocol Michael Kyba Book 2016 Springer Science+Business Media New York 2016 degene

DRAFT

Synergist Ablation as a Rodent Model to Study Satellite Cell Dynamics in Adult Skeletal Musclel activated by a stimulus, after which they proliferate, differentiate, and fuse into an existing myofiber or form a de novo myofiber. To study satellite cell dynamics in adult murine models, most studies utilize regeneration models in which the muscle is severely damaged and requires the participat

精密

NIL

Prophylaxis

Locale

独白

Isolation of Mouse Periocular Tissue for Histological and Immunostaining Analyses of the Extraocular of EOMs including their sparing in Duchenne muscular dystrophy have drawn a continuous interest, knowledge about these hard to reach muscles is still limited. The goal of this chapter is to provide detailed methods for the isolation and histological analysis of mouse EOMs. We first introduce in bri

肉体

Skeletal Muscle Tissue Clearing for LacZ and Fluorescent Reporters, and Immunofluorescence Staininghomeostasis. It is also a highly regenerative tissue that responds to damage in a highly intricate but stereotypic manner, with distinct spatial and temporal kinetics. Proper examination of this process requires one to look at the three-dimensional orientation of the cellular and subcellular compone

incredulity

Isolation, Culture, Functional Assays, and Immunofluorescence of Myofiber-Associated Satellite Cellsbers. Satellite cells reside in a niche between the basal lamina and sarcolemma of myofibers. Isolating single myofibers and their associated satellite cells provides a culture system that partially mimics the in vivo environment. We describe methods for isolating and culturing intact individual myo

Genome

Flow Cytometry and Transplantation-Based Quantitative Assays for Satellite Cell Self-Renewal and Dif and repopulating this new muscle with stem cells for future injury responses. For many questions relating to the physiological regulation of satellite cells, quantitative readouts of self-renewal and differentiation can be very useful. There is a particular need for a quantitative assay for satelli

Dealing

Noninvasive Tracking of Quiescent and Activated Muscle Stem Cell (MuSC) Engraftment Dynamics In Vivocell biology to devise strategies for treating failing muscles as seen in aging and muscle-related diseases. However, the common method used in assessing stem cell function in vivo is laborious, as it involves time-consuming immunohistological analyses by microscopy on serial cryo-sections of the mu