书目名称 | Skeletal Muscle Regeneration in the Mouse |
副标题 | Methods and Protocol |
编辑 | Michael Kyba |
视频video | |
概述 | Includes cutting-edge methods and protocols.Provides step-by-step details essential for reproducible results.Contains key notes and implementation advice from the experts |
丛书名称 | Methods in Molecular Biology |
图书封面 |  |
描述 | This volume focuseson the cell biology and physiology of skeletal muscle regeneration. This Bookis a collection of classic and cutting edge protocols optimized for mice, butin most cases adaptable to rat or other mammalian models, that will allow aninvestigator to develop and implement a research study on skeletal muscleregeneration. Chapters address the three major areas of study: provoking regeneration by inducing damage to muscle, analyzing theprogenitor cells of skeletal muscle, and quantifying overall muscle function. Subjects discussed include: inducing skeletal muscle injury byeccentric contraction; volumetric muscle loss; single myofiber isolation andculture; satellite cell transplantation; muscle clearing for whole mountimmunostaining; luciferase tracking of muscle stem cells; mitochondrialand mitophagy flux analysis; in vivo assessment of muscle contractility;force measurements on single isolated myofibers; and analysis of aerobicrespiration in intact skeletal muscle tissue by microplate respirometry. Written in the highlysuccessful .Methods in Molecular Biology .series format,chapters include introductions to each respective topic, lists of the necessarymaterials and rea |
出版日期 | Book 2016 |
关键词 | degeneration; tissue repair; skeletal muscle progenitor cells; skeletal muscle growth; regenerative ther |
版次 | 1 |
doi | https://doi.org/10.1007/978-1-4939-3810-0 |
isbn_softcover | 978-1-4939-8145-8 |
isbn_ebook | 978-1-4939-3810-0Series ISSN 1064-3745 Series E-ISSN 1940-6029 |
issn_series | 1064-3745 |
copyright | Springer Science+Business Media New York 2016 |