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Titlebook: Skeletal Muscle Regeneration in the Mouse; Methods and Protocol Michael Kyba Book 2016 Springer Science+Business Media New York 2016 degene

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书目名称Skeletal Muscle Regeneration in the Mouse
副标题Methods and Protocol
编辑Michael Kyba
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step details essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Skeletal Muscle Regeneration in the Mouse; Methods and Protocol Michael Kyba Book 2016 Springer Science+Business Media New York 2016 degene
描述This volume focuseson the cell biology and physiology of skeletal muscle regeneration. This Bookis a collection of classic and cutting edge protocols optimized for mice, butin most cases adaptable to rat or other mammalian models, that will allow aninvestigator to develop and implement a research study on skeletal muscleregeneration. Chapters address the three major areas of study: provoking regeneration by inducing damage to muscle, analyzing theprogenitor cells of skeletal muscle, and quantifying overall muscle function. Subjects discussed include: inducing skeletal muscle injury byeccentric contraction; volumetric muscle loss; single myofiber isolation andculture; satellite cell transplantation; muscle clearing for whole mountimmunostaining; luciferase tracking of muscle stem cells; mitochondrialand mitophagy flux analysis; in vivo assessment of muscle contractility;force measurements on single isolated myofibers; and analysis of aerobicrespiration in intact skeletal muscle tissue by microplate respirometry. Written in the highlysuccessful .Methods in Molecular Biology .series format,chapters include introductions to each respective topic, lists of the necessarymaterials and rea
出版日期Book 2016
关键词degeneration; tissue repair; skeletal muscle progenitor cells; skeletal muscle growth; regenerative ther
版次1
doihttps://doi.org/10.1007/978-1-4939-3810-0
isbn_softcover978-1-4939-8145-8
isbn_ebook978-1-4939-3810-0Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2016
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Skeletal Muscle Tissue Clearing for LacZ and Fluorescent Reporters, and Immunofluorescence Stainingscent reporters and immunofluorescence staining. Using this technique, we are able to visualize LacZ reporters more effectively in deep tissue samples, and to perform fluorescent imaging with a depth greater than 1700 μm.
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Myogenic Progenitors from Mouse Pluripotent Stem Cells for Muscle Regeneration their relevance in both cell therapy and disease modeling, in this chapter we describe the protocol to derive myogenic progenitors from murine ES cells followed by their intramuscular delivery in a murine muscular dystrophy model.
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Noninvasive Tracking of Quiescent and Activated Muscle Stem Cell (MuSC) Engraftment Dynamics In Vivosive tracking of engrafted stem-cell function in vivo in real-time. This assay system enables longitudinal studies in the same mice over time and reveals parameters, not feasible by traditional analysis, such as the magnitude and dynamics of engrafted muscle stem cell expansion in vivo in response to a particular drug treatment or muscle injury.
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