Titlebook: Single Molecule Microscopy in Neurobiology; Nobuhiko Yamamoto,Yasushi Okada Book 2020 Springer Science+Business Media, LLC, part of Spring

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Live-Cell Single-Molecule Imaging with Optogenetics Reveals Dynamics of a Neuronal Activity-Dependel functions. A fine-tuned single-molecule imaging technique allows us to study the movement of transcription factors in the cell nucleus. Our technique combined with optogenetics enables us to reveal neuronal activity-dependent dynamics of transcription factors in living cortical neurons. Here, we d

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Approaching Protein-Protein Interactions in Membranes Using Single-Particle Tracking and Packing Coceable changes in diffusion behavior such as a slowdown or immobilization by formation of complexes. As a consequence, the analysis of transitions between diffusive behaviors can provide effective rate constants of molecular interactions. Tracking the positions of labeled single molecules, i.e., usi

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Arable

Multipolarization Dark-Field Imaging of Single Endosomes in Microfluidic Neuronal Culture for Simul teams of molecular motors. How these motors are regulated remains poorly understood. In part, this is due to the high variability between individual endosomes. Conventional imaging approaches also only track the position of endosomes, effectively measuring one variable in a team of several motors.

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Nanoscale Molecular Imaging of Presynaptic Active Zone Proteins in Cultured Hippocampal Neurons,roteins reside. The composition and nanoscale spatial organization of the active zone proteins are crucial determinants of presynaptic functions, and variety in the composition and organization may account for wide diversity of central synapses. Here, we describe stochastic optical reconstruction mi

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Three-Dimensional Super-Resolution Imaging of the Cytoskeleton in Hippocampal Neurons Using Selectil structures in cellular and molecular biology. A number of super-resolution optical microscopy techniques have been reported; however, several challenges such as the use of high-activation sources resulting in photobleaching and photodamage effects, restrictions in three-dimensional imaging, long d