Titlebook: Single Molecule Microscopy in Neurobiology; Nobuhiko Yamamoto,Yasushi Okada Book 2020 Springer Science+Business Media, LLC, part of Spring

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Single-Molecule Imaging of Intracellular Transport in Neurons and Non-neuronal Cells: From Microscof kinetics and dynamics of single molecules in living cells. However, sample preparation is equally important for the successful single-molecule imaging. Here, we describe the equipment of TIRFM as well as the procedures for sample preparations for the single-molecule imaging in living cultured cells.

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Synaptic Function and Neuropathological Disease Revealed by Quantum Dot-Single-Particle Tracking, molecules reflects the condition of neurons in pathological disease states. In this chapter, we describe the latest, simple QD-SPT technique, which is feasible with epifluorescence microscopy and dissociated cell cultures.

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Book 2020patterns. Chapters in this book cover topics such as in vivo single-molecule tracking of voltage-gated calcium channels with split-fluorescent proteins in CRISPR-engineering C. elegans; protein-protein interactions in membranes using single particle tracking; neuropathological diseases revealed by q

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APNEA

Live-Cell Single-Molecule Imaging with Optogenetics Reveals Dynamics of a Neuronal Activity-Dependee combined with optogenetics enables us to reveal neuronal activity-dependent dynamics of transcription factors in living cortical neurons. Here, we describe the detailed experimental procedures to study the transcriptional activity with physiological stimulation in living CNS neurons.

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Single-Molecule Imaging of Intracellular Transport in Neurons and Non-neuronal Cells: From Microsconsport a variety of cellular components. Translocation of motor proteins would be highly regulated, since various cargos are transported to their appropriate destinations. Single-molecule investigation of dynamic movement of motor proteins in living cells has enabled us to explore these regulatory m

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In Vivo Single-Molecule Tracking of Voltage-Gated Calcium Channels with Split-Fluorescent Proteins and ex vivo systems via biochemical and electrophysiological methods. With the emergence of single-molecule (SM) fluorescence microscopy techniques, it is now possible to characterize the molecular organization and the biophysical dynamics of ion channels in cells with precisions on the order of a f

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