Titlebook: Single Cell Protein Analysis; Methods and Protocol Anup K. Singh,Aarthi Chandrasekaran Book 2015 Springer Science+Business Media New York 2

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1064-3745 ation advice from the experts.Includes supplementary materia.This volume highlights recent developments in flow cytometry, affinity assays, imaging, mass spectrometry, microfluidics and other technologies that enable analysis of proteins at the single cell level. The book also includes chapters cove

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Single-Cell Western Blotting,ed simultaneously, and multiple protein targets of interest are investigated. After PAGE separation, photo-immobilization of all proteins to the gel allows for antibody probing and lends to the archival quality of the scWestern assay where new proteins targets can be investigated months after the initial separations are performed.

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Microfluidics-Enabled Enzyme Activity Measurement in Single Cells,ets significantly improve enzyme extraction from human cells or microorganisms and result in faster reaction kinetics. Taken together, the described protocol is expected to open up new possibilities in the single-cell research, particularly for the elucidation of heterogeneity in a population of cells.

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Book 2015enable analysis of proteins at the single cell level. The book also includes chapters covering a suite of biochemical and biophysical methods capable of making an entire gamut of proteomic measurements, including analysis of protein abundance or expression, protein interaction networks, post-transla

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Microfluidic Proximity Ligation Assay for Profiling Signaling Networks with Single-Cell Resolution,ed cell stimulation and fixation, PLA implementation, and computational image analysis in order to achieve single-cell resolution. As a proof of concept, we studied the phosphorylation of Akt in response to stimulation with platelet-derived growth factor.

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Photocleavable DNA Barcoding Antibodies for Multiplexed Protein Analysis in Single Cells,zation technology for detection, similar to what is commonly done for nucleic acid readouts. This protocol offers a simple method for multiplexed protein detection using 100+ antibodies and can be performed on clinical samples as well as single cells.