Titlebook: Single Cell Protein Analysis; Methods and Protocol Anup K. Singh,Aarthi Chandrasekaran Book 2015 Springer Science+Business Media New York 2

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Microfluidic Image Cytometry for Single-Cell Phenotyping of Human Pluripotent Stem Cells,ed serum- and feeder-free culture conditions. This microfluidic platform, combined with image cytometry, enables the systematic analysis of multiple simultaneously detected marker expression in individual cells, for screening of various chemically defined media across hPSC lines, and the study of ph

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Characterizing Phenotypes and Signaling Networks of Single Human Cells by Mass Cytometry,periments routinely measure 25–35 features of each cell in primary human tissue samples. The relative ease with which a novice user can generate a large amount of high quality data and the novelty of the approach have created a need for example protocols, analysis strategies, and datasets. In this c

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Imaging and Mapping of Tissue Constituents at the Single-Cell Level Using MALDI MSI and Quantitativroteins). With the advent of antibody-labeling, immunostaining (fluorescein and rhodamine for fluorescent labeling) and immunohistochemistry (DAB and hematoxylin), it became possible to identify specific immunological targets in cells and tissue preparations. Technical advances, including the develo

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Dynamics and Interactions of Individual Proteins in the Membrane of Single, Living Cells,cells. Receptor–ligand interactions are of particular interest for improving our understanding of cell signaling networks in a variety of applications. Here, we describe methods for fluorescently labeling individual receptors and their ligands, conducting single-molecule TIRF microscopy of receptors

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Microfluidic Chemical Cytometry for Enzyme Assays of Single Cells,rticular, single-cell measures of protein levels are complemented by single-cell measurements of the activity of these proteins. Microfluidic assays of enzyme activity at the single-cell level combine moderate to high throughput with low dead volumes and the potential for automation. Herein, we desc

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Quantitative Detection of Nucleocytoplasmic Transport of Native Proteins in Single Cells,imaging and subcellular-fractionation-based techniques that do not generate information on a large cell population with single-cell resolution. Although special flow cytometric tools such as imaging flow cytometry may generate single-cell data on processes such as nucleocytoplasmic transport, such e