Titlebook: Single Cell Diagnostics; Methods and Protocol Alan Thornhill Book 2007 Humana Press 2007 DNA.FISH.Fluoreszenz in situ-Hybridisierung.Microa

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Gender Determination and Detection of Aneuploidy in Single Cells Using DNA Array-Based Comparative s hindered by the time required to perform the analysis. DNA microarrays manufactured to date are not able to analyze the very limited amount of genetic material in a single cell. We have developed a DNA Microarray-Comparative Genomic Hybrydization (CGH) approach, which is capable of screening all 2

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1543-1894 om single cells.Microarrays for analyzing multiple gene sequThe aim of Single Cell Diagnostics: Methods and Protocols is for all readers to extend their knowledge and expertise in analysis of single cells. The book starts with laser assisted cell collection, non-invasive assessment of single cells a

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Book 2007eases as real-time quantitative PCR, isothermal whole genome amplification, comparative genomic hybridization, real-time gene expression analysis and the production of RNA and cDNA libraries are covered. The book closes with the application of customized microarrays to the study of single cells.

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Laser Capture Microdissection for Analysis of Single Cells,ng the reproducible analysis of gene expression from 1 to 10 cells. These methods show the utility of immuno-LCM as well as LCM combined with RT-PCR for cellular and molecular studies of gene expression.

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Efficient Isothermal Amplification of the Entire Genome from Single Cells, multiple displacement amplification (MDA) offers approx 5 million-fold amplification with fidelity, apparently sidestepping the limitation of a single cell, and is sufficient for use in most off-the-shelf molecular tests. This chapter describes an optimized MDA protocol for the preparation of genomic DNA from single fibroblasts.

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