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Titlebook: Single Cell Diagnostics; Methods and Protocol Alan Thornhill Book 2007 Humana Press 2007 DNA.FISH.Fluoreszenz in situ-Hybridisierung.Microa

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书目名称Single Cell Diagnostics
副标题Methods and Protocol
编辑Alan Thornhill
视频video
概述Up-to-date, clinically applicable, validated protocols for single-cell diagnostics.Whole genome amplification to create starting material from single cells.Microarrays for analyzing multiple gene sequ
丛书名称Methods in Molecular Medicine
图书封面Titlebook: Single Cell Diagnostics; Methods and Protocol Alan Thornhill Book 2007 Humana Press 2007 DNA.FISH.Fluoreszenz in situ-Hybridisierung.Microa
描述The aim of Single Cell Diagnostics: Methods and Protocols is for all readers to extend their knowledge and expertise in analysis of single cells. The book starts with laser assisted cell collection, non-invasive assessment of single cells and moves through the techniques of standard fluorescence in situ hybridization and polymerase chain reaction (PCR) As the reader moves through the book, the scope and complexity of each technique gradually increases as real-time quantitative PCR, isothermal whole genome amplification, comparative genomic hybridization, real-time gene expression analysis and the production of RNA and cDNA libraries are covered. The book closes with the application of customized microarrays to the study of single cells.
出版日期Book 2007
关键词DNA; FISH; Fluoreszenz in situ-Hybridisierung; Microarray; Mutation; PCR; Quantitative PCR; Termination; chr
版次1
doihttps://doi.org/10.1007/978-1-59745-298-4
isbn_softcover978-1-61737-654-2
isbn_ebook978-1-59745-298-4Series ISSN 1543-1894 Series E-ISSN 1940-6037
issn_series 1543-1894
copyrightHumana Press 2007
The information of publication is updating

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Jill L. Maron MD, MPH,Kirby L. Johnson PhD,Diana W. Bianchi MDlogische Einzelleistungen: Die Tätigkeit der Leber oder des Darms, sondern auch die Gesamtleistung des Körpers zu verändern, zu fördern oder zu hemmen. Das wäre nicht möglich, wenn nicht ein morphologischer Zusammenhang aller Teile des Körpers bestünde. Der aber ist gewährleistet durch drei Gewebe,
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James Adjaye PhDngen selten, insgesamt betreffen sie aber mehr als 1% der Bevölkerung. Für die genetische Beratung besitzen sie dreifache Bedeutung: Erstens ist es wichtig, eine betroffene Familie, ungeachtet der Seltenheit des Merkmals, korrekt zu beraten. Zweitens können Verwechselungen dieser seltenen, hoch risk
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Fluorescence , Hybridization on Single Cells,trategies for PGD for sex determination and chromosome rearrangements. These methods are assessment of reproductive risks, the selection of suitable probes for interphase FISH, spreading techniques for blastomere nuclei, and . hybridization and signal scoring using directly labeled and indirectly labeled probes.
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Noninvasive Metabolic Assessment of Single Cells,sment of viability, analyses not only must be sensitive enough to be employed at the single cell level, but also noninvasive. Ultramicrofluorescence is a technique that fulfills both criteria. By using specially prepared constriction pipets, created on a microforge and calibrated with tritiated wate
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