Titlebook: Sialo-Xenoantigenic Glycobiology; Molecular Glycobiolo Kwon-Ho Song,Cheorl-Ho Kim Book 2013 Springer-Verlag Berlin Heidelberg 2013 Carbohyd

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Screening for Xenoantigenic Determinants Formed by Sialyltransferases,(hST3Gal II) and human .-2, 6- sialyltransferase (hST6Gal I, hST6GalNAc IV). The transfected cells were screened by Western blot analysis and Lectin blot analysis. The cytotoxicity and xenoreactivity of the PK 15 cell lines were examined by performing the LDH cytotoxicity assay and FACS analysis. Fr

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Identification of Two Alternative Promoters of the Pig CMP-,-Acetylneuraminic Acid Hydroxylase Gene-2I cells compared to thePK15 cells, where the P1 promoter has only basal activity, suggestive of an intestine-specific promoter. Furthermore, our results demonstrate that the Sp1 transcription factor is akey regulatory element important for the regulation of . expression.

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Screening for Xenoantigenic Determinants Formed by Sialyltransferases,erefore, our results suggest that alteration of glycosylation-pattern by sialyltransferases including hST3Gal II and hST6GalNAc IV gene results in increase of human serum-mediated cytotoxicity to pig kidney cells and the increased susceptibilities to cytotoxicity may result from the increased human

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2195-3546 t chapter the screening for pig glycosyltransferase genes for xenoantigens is presented. In the chapter II to IV the cloning, characterization, and investigation of the regulatory mechanism of t978-3-642-43443-3978-3-642-34094-9Series ISSN 2195-3546 Series E-ISSN 2195-3554

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