Titlebook: Shiga Toxin-Producing E. coli; Methods and Protocol Stephanie Schüller,Martina Bielaszewska Book 2021 Springer Science+Business Media, LLC,

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Thin-Layer Chromatography in Structure and Recognition Studies of Shiga Toxin Glycosphingolipid Rec a fatty acid (mostly C16–C24) and a sugar moiety harboring to a great extent one to five monosaccharides. GSLs of the globo-series are well-recognized receptors of Shiga toxins (Stxs) released by Stx-producing . (STEC). Receptors for the Stx subtypes Stx1a and Stx2a are globotriaosylceramide (Gb3Ce

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Identification of Nanobodies Blocking Intimate Adherence of Shiga Toxin-Producing , to Epithelial CTEC) in the intestine of the patient and also in the bovine reservoir, thereby minimizing the risk of STEC contamination in the food chain. Of particular interest are recombinant single-domain Ab fragments called nanobodies (Nbs) derived from the variable domain of camelid heavy chain-only antibodie

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,Human Epithelial Stem Cell-Derived Colonoid Monolayers as a Model to Study Shiga Toxin-Producing ,–gy and pathologies as they can be propagated indefinitely and preserve the genetic signature of the donor and the gut segment specificity in culture. Here we describe human stem cell-derived colonoid monolayers as a reliable and reproducible model to study Shiga toxin-producing . (STEC) infection an

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Interaction of Bovine Lymphocytes with Products of Shiga Toxin-Producing ,d proliferation and pro-inflammatory cytokine synthesis. Here we describe how to isolate lymphocyte subsets from bovine peripheral blood as well as methods that we have used to study the effects of STEC products on lymphocyte proliferation and cytokine production. We also describe an assay that allo

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Infant Rabbit Model for Studying Shiga Toxin-Producing ,toxin-producing . (STEC). The optimal model may differ depending on what aspects of pathogen biology, disease progression, or host response are under study. Here, we provide detailed protocols for the infant rabbit model of STEC, which largely reproduces the intestinal disease seen following natural

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Lysogenized with a Shiga Toxin-Producing Phage: A Murine Model for Shiga Toxin-Producing , Infectiepithelium, characterized by effacement of microvilli and the formation of actin “pedestals” beneath intimately attached bacteria. In addition, STEC are lysogenized with a phage that, upon induction, can produce potent Shiga toxins (Stx), potentially leading to both hemorrhagic colitis and hemolytic