Titlebook: Shiga Toxin-Producing E. coli; Methods and Protocol Stephanie Schüller,Martina Bielaszewska Book 2021 Springer Science+Business Media, LLC,

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Shiga Toxin-Producing E. coli978-1-0716-1339-9Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Identification of Shiga Toxin-Producing , Outbreaks Using Whole Genome Sequencing,iate them from sporadic cases. Here, we describe an optimized protocol to efficiently determine the genome sequences of STEC using short read Illumina technology and provide information on helpful tools for the subsequent bioinformatic analysis.

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https://doi.org/10.1007/978-1-0716-1339-9STEC; Foodborne pathogen; Virulence factors; Human pathogenesis; Clinical diagnosis

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1064-3745 ation advice from the experts.This volume provides a comprehensive outline of the current methods used to detect, characterize, and investigate Shiga toxin-producing .E. coli.  (STEC) and Shiga toxins. Written in the highly successful .Methods in Molecular Biology .series format, chapters include in

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Integrated Approach for the Diagnosis of Shiga Toxin-Producing , Infections in Humans,tions include both direct isolation of the STEC from stool samples and the identification of indirect evidences based on molecular, phenotypic, and serological applications. Here, the procedures in use at the Italian Reference Laboratory for . infections are described.

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