Titlebook: Selective Autophagy; Methods and Protocol Ioannis P. Nezis Book 2024 The Editor(s) (if applicable) and The Author(s), under exclusive licen

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In Silico Assessment of LIR Motif-Containing Proteins Binding to ATG8-Family Proteins,mily proteins. Here, we present a detailed methodology for the in silico evaluation of potential binding between LIR motif-containing proteins and ATG8-family proteins. We visualize AlphaFold-predicted protein complexes using PyMOL to assess potential interactions, providing an effective computation

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High-Throughput Screening for LC3/GABARAP Binders Utilizing the Fluorescence Polarization Assay,ecules is important for a detailed understanding of selective autophagy mechanisms and for the design of potential Atg8 inhibitors that affect the autophagy processes in cells. The fluorescence polarization (FP) assay is a rapid, cost-effective, and robust method that provides affinity and selectivi

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Thermodynamic Characterization of LC3/GABARAP:Ligand Interactions by Isothermal Titration Calorimetides information on the stoichiometry, affinity, and thermodynamic driving forces of interactions. This chapter exemplifies the use of ITC to investigate interactions between human autophagy modifiers (LC3/GABARAP proteins) and their interaction partners, the LIR motif-containing sequences. The purp

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Determination of hATG8 Binding Selectivity of AIM (Autophagy-Interacting Motif) Peptides Using Natio their biological roles, and in the case of disease-associated AIM mutations, potential pathophysiological mechanisms. Given the sometimes small differences in affinity for an individual AIM amongst the six hATG8 proteins, establishing AIM preferences can be experimentally challenging. We describe

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Methods in Molecular Biologyhttp://image.papertrans.cn/s/image/864309.jpg

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https://doi.org/10.1007/978-1-0716-4067-8reticulophagy; ribophagy; Drosophila; zebrafish; pexophagy; non-mammalian model; C; elegans

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