Titlebook: Selective Autophagy; Methods and Protocol Ioannis P. Nezis Book 2024 The Editor(s) (if applicable) and The Author(s), under exclusive licen

重要

书目名称Selective Autophagy影响因子(影响力)




书目名称Selective Autophagy影响因子(影响力)学科排名




书目名称Selective Autophagy网络公开度




书目名称Selective Autophagy网络公开度学科排名




书目名称Selective Autophagy被引频次




书目名称Selective Autophagy被引频次学科排名




书目名称Selective Autophagy年度引用




书目名称Selective Autophagy年度引用学科排名




书目名称Selective Autophagy读者反馈




书目名称Selective Autophagy读者反馈学科排名

Condescending

Synthetic Dimerization Approaches for In Vivo Studies in ,e describe two chemically induced dimerization systems in the model organism . Using the autophagy pathway as an example, we show how these approaches can be used to dissect molecular events in cells.

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Assays to Study Mitophagy in Plants,ial marker lines followed by image analysis, chemical inhibitor treatment, and plant phenotype studies. These methods can be used in combination to identify the putative mitophagy regulators and understand their functions in mitochondrial-related activities in plants.

积极词汇

Fluorescence Microscopy and Immunoblotting for Mitophagy in Budding Yeast,pregulated under prolonged respiration or nutrient starvation, anchored on the surface of mitochondria, and activated to recruit the autophagy machinery for the formation of autophagosomes surrounding mitochondria. In this chapter, we provide protocols to assess Atg32-mediated mitophagy using fluorescence microscopy and immunoblotting.

新义

CRANK

Studying Selective Autophagy of Protein Aggregates Using Particles Induced by Multimerization (PIMs use of pH-sensitive fluorescent proteins, such as GFP or mKeima, the PIM assay allows for direct visualization of aggregate clearance in cells. Here, we describe a protocol for the use of the PIM assay to study aggrephagy in live and fixed cells.

Control-Group

High-Throughput Screening for LC3/GABARAP Binders Utilizing the Fluorescence Polarization Assay,dition, a case study on peptide interaction with human Atg8 proteins (LC3/GABARAPs) is described. Finally, data analysis and quality control of FP assays are discussed for the proper calculation of . values for the measured compounds.

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1064-3745 ation advice from the experts.This volume presents the latest techniques used by researchers to study selective autophagy in a broad range of organisms such as yeast, plants, worms, fruit flies, and mammals. The chapters in this book cover several types of selective autophagy such as mitophagy, aggr