Titlebook: SUMO; Methods and Protocol Manuel S. Rodriguez Book 2016 Springer Science+Business Media New York 2016 small ubiquitin-related modifier SUM

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Concepts and Methodologies to Study Protein SUMOylation: An Overviewamic reversible system. New conceptual frameworks suggested by these findings have motivated the development of new methodologies to study pre- and post-SUMOylation events in vitro and in vivo, using distinct model organisms. Here we summarize some of the new developments and methodologies in the fi

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Analysis of SUMOylated Proteins in Cells and In Vivo Using the bioSUMO Strategy chromatography under stringent conditions and with very low background. The bioSUMO approach can be used to validate whether a specific protein is modified, or used to analyze an entire SUMO subproteome. If coupled to quantitative proteomics methods, it may reveal how the SUMO landscape changes wit

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Label-Free Identification and Quantification of SUMO Target Proteinse or cell labeling techniques. The method described here allows for identification of >1000 SUMO target proteins, and characterization of their SUMOylation dynamics, without requiring sample fractionation. The purification procedure, starting from total lysate, can be performed in ~4 days.

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Michael J. Matunis,Manuel S. RodriguezFlechsig Institut for Brain Research, University of Leipzig,.Active touch perception, or haptic perception, is of primary importance for the planning, direction and execution of everyday actions. This complex human sensory system is gaining ever more importance for various scientific disciplines as

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Flechsig Institut for Brain Research, University of Leipzig,.Active touch perception, or haptic perception, is of primary importance for the planning, direction and execution of everyday actions. This complex human sensory system is gaining ever more importance for various scientific disciplines as