Titlebook: SUMO; Methods and Protocol Manuel S. Rodriguez Book 2016 Springer Science+Business Media New York 2016 small ubiquitin-related modifier SUM

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Ivo A. Hendriks,Alfred C. O. Vertegaalcies still represent the most important of all public health problems. The ability of these infectious agents to jump across species barriers to infect humans presents a formidable challenge to our survival instincts—not to mention our public health infrastructure. In the United States, we are somew

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Elisa Aguilar-Martínez,Andrew D. Sharrocks As a teacher of Environmental Health since the late 1950s, I have witnessed the many twists and turns that have marked the latter half of the century, and have had to seek continuously to explain these phenomena to my students in some rational manner. We have witnessed the following and more: great

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Tasneem Bawa-Khalfemost easterly point of Mindanao to the Balabac Island situated at the southern tip of Palawan in the west, the Philippine Archipelago comprises a triangular area of around 1.3 million square kilometres of the Western Pacific. Broken up in part by Tertiary folds and partly by the post-glacial rise in

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The Regulation of Chromatin by Dynamic SUMO Modificationsross chromatin reveals an important function for SUMO in controlling transcription, particularly of genes involved in protein synthesis. These newly appreciated patterns of genome-wide sumoylation will inform more directed studies aimed at analyzing how the dynamics of gene expression are controlled by posttranslational SUMO modification.

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Reconstitution of the Recombinant RanBP2 SUMO E3 Ligase Complex, the SUMO E2-conjugating enzyme Ubc9, RanGAP1, and SUMO1, and we provide a protocol for quantitative SUMOylation of RanGAP1. Finally, we present details for the assembly and final purification of the catalytically active RanBP2/RanGAP1*SUMO1/Ubc9 complex.

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Production and Purification of Recombinant SUMOylated Proteins Using Engineered Bacteriarates, the efficiency of in vitro SUMOylation is too low to obtain sufficient amounts of their SUMOylated forms for biochemical studies. Here, we describe a detailed protocol to purify large amounts of recombinant SUMOylated proteins using bacteria modified to express His-tagged SUMO as well as the SUMO-activating and -conjugating enzymes.

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