Titlebook: SH2 Domains; Methods and Protocol Kazuya Machida,Bernard A. Liu Book 2017 Springer Science+Business Media LLC 2017 receptor tyrosine kinase

Cabinet

Creation of Phosphotyrosine Superbinders by Directed Evolution of an SH2 Domainlay technology. The superbinders are stable and can be easily produced in . in large quantities. The strategy presented here may also be applied to other protein domains to generate domain variants with markedly enhanced affinities for a specific post-translational modification.

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BANAL

978-1-4939-8295-0Springer Science+Business Media LLC 2017

内向者

SH2 Domains978-1-4939-6762-9Series ISSN 1064-3745 Series E-ISSN 1940-6029

Ballad

Kazuya Machida,Bernard A. LiuIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts

草率女

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carbohydrate

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青春期

Expression and Purification of SH2 Domains Using Baculovirus Expression Systeme insoluble in bacteria, baculovirus-insect cell expression systems can be an alternative to produce soluble and functionally active proteins. We describe a protocol for cloning and purification of GST-tagged SH2 domains using the Bac-to-Bac baculovirus expression system.