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Titlebook: Root Development; Methods and Protocol Daniela Ristova,Elke Barbez Book 2018 Springer Science+Business Media, LLC 2018 Environmental change

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Book 2018iology as well as for use in plant species other than .Arabidopsis thaliana.. The collection covers methods ranging from genetic screens, phenotypic analysis, and cell biology methods to systems biology tools and genome-wide approaches. The collection contains a range of complexity from fundamental
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Genetic and Phenotypic Analysis of Lateral Root Development in cells produced within a root. Hormonal misbalances and mutations affect LRP morphogenesis significantly, and the evaluation of LRP abnormalities is addressed as well. Finally, we deal with various molecular markers that can be used for genetic and phenotypic analyses of LR development.
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Root Gravitropism: Quantification, Challenges, and Solutionses a “particle-filtering algorithm with a graph-based method” to trace the center line of a root and can be adopted for the analysis of several root parameters such as length, curvature, and stimulus from original root traces.
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Light Sheet Fluorescence Microscopy Optimized for Long-Term Imaging of Arabidopsis Root Developmentzed for tracking and 3D scanning of . root tips grown vertically in physiological conditions. The system is relatively inexpensive and with minimal footprint; hence it is well suited for laboratories of any size.
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Histological Profiling Over Time to Optimize Root Cell Type-Specific Reporter Lines for Cell Sortingth and width of the root using the model legume . as an example. In order to deal with the fact that plant cell walls are highly autofluorescent, we also describe the usage of confocal microscopy to conduct a lambda scan to discriminate autofluorescence from marker molecule expression.
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