Titlebook: Root Development; Methods and Protocol Daniela Ristova,Elke Barbez Book 2018 Springer Science+Business Media, LLC 2018 Environmental change

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Light Sheet Fluorescence Microscopy Optimized for Long-Term Imaging of Arabidopsis Root Developmentn, with minimal photodamage. Here, we describe in detail both the hardware and the software elements of a live imaging method based on LSFM and optimized for tracking and 3D scanning of . root tips grown vertically in physiological conditions. The system is relatively inexpensive and with minimal fo

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Long-Term In Vivo Imaging of Luciferase-Based Reporter Gene Expression in Arabidopsis Rootsdevelopmental pattern to mine the soil for water and nutrients while avoiding patches of soil that contain biotic and abiotic stress agents. Because most developmental processes are taking place gradually while roots are growing, it is often difficult to correlate gene expression events with specifi

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Cortical Cell Length Analysis During Gravitropic Root Growthhe root tip angle toward gravity and, hence, does not directly assess the actual differential growth process. Here, we describe the cortical cell length as a parameter to quantify cell elongation during the gravitropic response, using median longitudinal confocal sections. This analysis depicts that

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