Titlebook: Ribozymes; Methods and Protocol Robert J Scarborough,Anne Gatignol Book 2021 Springer Science+Business Media, LLC, part of Springer Nature

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1064-3745 ation advice from the experts.This volume provides protocols designed to study the function and the structure of diverse ribozymes. Chapters guide readers through different techniques to identify and characterize new ribozymes and methods to use ribozymes to alter the function of CRISPR-based guide

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Co-transcriptional Analysis of Self-Cleaving Ribozymes and Their Ligand Dependenceiochemical study and understanding of these molecules. Here we describe a co-transcriptional assay that allows the analysis of self-cleaving ribozymes in different reaction conditions and in the presence of desired ligands and/or cofactors. Utilizing a standard T7 RNA polymerase in vitro transcripti

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Characterization and Optimization of a Deoxyribozyme with a Short Left Binding Armzymes normally contain two substrate-binding arms and a central catalytic core region. Here, we describe the systematic characterization and optimization of an RNA-cleaving deoxyribozyme with an unusually short left binding arm, and its special sequence requirement for its optimal catalytic activity

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