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Titlebook: Ribozymes; Methods and Protocol Robert J Scarborough,Anne Gatignol Book 2021 Springer Science+Business Media, LLC, part of Springer Nature

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发表于 2025-3-21 19:47:54 | 显示全部楼层 |阅读模式
书目名称Ribozymes
副标题Methods and Protocol
编辑Robert J Scarborough,Anne Gatignol
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Ribozymes; Methods and Protocol Robert J Scarborough,Anne Gatignol Book 2021 Springer Science+Business Media, LLC, part of Springer Nature
描述.This volume provides protocols designed to study the function and the structure of diverse ribozymes. Chapters guide readers through different techniques to identify and characterize new ribozymes and methods to use ribozymes to alter the function of CRISPR-based guide RNAs, AgoshRNAs and aptamers or to study RNA capping and long non-coding RNAs. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls... Authoritative and cutting-edge, .Ribozymes: Methods and Protocols .aims to help in accelerating ribozyme research and inspiring others to develop new methods to study ribozyme structure and function..
出版日期Book 2021
关键词hammerhead ribozymes; human microbiome; archaeal genomes; comparative genomics; RNase P
版次1
doihttps://doi.org/10.1007/978-1-0716-0716-9
isbn_softcover978-1-0716-0718-3
isbn_ebook978-1-0716-0716-9Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2021
The information of publication is updating

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发表于 2025-3-21 22:13:03 | 显示全部楼层
Cloning and Detection of Genomic Retrozymes and Their circRNA Intermediateser retrotransposons, transcription of retrozymes in vivo leads to the formation and accumulation of circular RNAs, which can be readily detected by RNA blotting. In this chapter, we describe the procedures needed to carry out the cloning of genomic retrozymes, and to detect by northern blot their circular RNA retrotransposition intermediates.
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Book 2021 on troubleshooting and avoiding known pitfalls... Authoritative and cutting-edge, .Ribozymes: Methods and Protocols .aims to help in accelerating ribozyme research and inspiring others to develop new methods to study ribozyme structure and function..
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Conjugation as a Highly Sensitive Assay to Study Group II Intron Splicing In Vivomine the splicing competence of various Ll.LtrB mutants, whereas the splicing selection/conjugation assay was established to isolate splicing-proficient variants from a randomly generated bank of mutated introns.
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Co-transcriptional Analysis of Self-Cleaving Ribozymes and Their Ligand Dependenceication or additional preparation steps, such as refolding procedures. Furthermore, because the transcripts are not denatured, this assay likely yields RNAs in conformations relevant to co-transcriptionally folded species in vivo.
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