书目名称 | Riboregulator Design and Analysis | 编辑 | James Chappell,Melissa K. Takahashi | 视频video | | 概述 | Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts | 丛书名称 | Methods in Molecular Biology | 图书封面 |  | 描述 | This collection explores the latest advances in riboregulators, such as RNA-only systems and ribonucleoprotein systems, and provides detailed techniques to study, evolve, and design them. Beginning with a set of chapters focused on the design and application of small RNA (sRNA) regulator systems, the book continues with sections on techniques to create switchable riboregulator systems known as riboswitches, technologies that leverage RNA-guided CRISPR-Cas systems to edit the epigenome, control gene expression, and create diagnostics, as well as computational and experimental techniques to investigate the sequence-structure-function relationship of RNA systems that can both advance fundamental understanding and rational design of riboregulators. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, andtips on troubleshooting and avoiding known pitfalls. .Authoritative and practical, .Riboregulator Design and Analysis. is an ideal guide for scientists and engineers interested in the design and application | 出版日期 | Book 2022 | 关键词 | Synthetic RNA regulatory mechanisms; Ribonucleoprotein systems; RNA-guided CRISPR-Cas; Riboswitches; Sma | 版次 | 1 | doi | https://doi.org/10.1007/978-1-0716-2421-0 | isbn_softcover | 978-1-0716-2423-4 | isbn_ebook | 978-1-0716-2421-0Series ISSN 1064-3745 Series E-ISSN 1940-6029 | issn_series | 1064-3745 | copyright | The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines |
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