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Titlebook: Riboregulator Design and Analysis; James Chappell,Melissa K. Takahashi Book 2022 The Editor(s) (if applicable) and The Author(s), under ex

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发表于 2025-3-21 18:01:30 | 显示全部楼层 |阅读模式
书目名称Riboregulator Design and Analysis
编辑James Chappell,Melissa K. Takahashi
视频video
概述Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Riboregulator Design and Analysis;  James Chappell,Melissa K. Takahashi Book 2022 The Editor(s) (if applicable) and The Author(s), under ex
描述This collection explores the latest advances in riboregulators, such as RNA-only systems and ribonucleoprotein systems, and provides detailed techniques to study, evolve, and design them. Beginning with a set of chapters focused on the design and application of small RNA (sRNA) regulator systems, the book continues with sections on techniques to create switchable riboregulator systems known as riboswitches, technologies that leverage RNA-guided CRISPR-Cas systems to edit the epigenome, control gene expression, and create diagnostics, as well as computational and experimental techniques to investigate the sequence-structure-function relationship of RNA systems that can both advance fundamental understanding and rational design of riboregulators. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, andtips on troubleshooting and avoiding known pitfalls. .Authoritative and practical, .Riboregulator Design and Analysis. is an ideal guide for scientists and engineers interested in the design and application
出版日期Book 2022
关键词Synthetic RNA regulatory mechanisms; Ribonucleoprotein systems; RNA-guided CRISPR-Cas; Riboswitches; Sma
版次1
doihttps://doi.org/10.1007/978-1-0716-2421-0
isbn_softcover978-1-0716-2423-4
isbn_ebook978-1-0716-2421-0Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
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Riboregulator Design and Analysis978-1-0716-2421-0Series ISSN 1064-3745 Series E-ISSN 1940-6029
发表于 2025-3-22 02:05:20 | 显示全部楼层
James Chappell,Melissa K. TakahashiIncludes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
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Design, Characterization, and Application of Targeted Gene Activation in Bacteria Using a Modular Cerest. Here, we describe a protocol for designing, characterizing, and applying the modular CRISPRa system for gene activation in .. We first focus on how to identify activating sites upstream of promoters and the cloning of the targeting sgRNA. We then describe how to perform a fluorescence experim
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