Titlebook: Riboregulator Design and Analysis; James Chappell,Melissa K. Takahashi Book 2022 The Editor(s) (if applicable) and The Author(s), under ex

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1064-3745 expertsThis collection explores the latest advances in riboregulators, such as RNA-only systems and ribonucleoprotein systems, and provides detailed techniques to study, evolve, and design them. Beginning with a set of chapters focused on the design and application of small RNA (sRNA) regulator sys

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Harnessing CRISPR-Cas9 for Epigenetic Engineering,to transiently transfecting the gRNA plasmid and the CRISPR/dCas9-based epigenetic effector and finalizing with chromatin immunoprecipitation (ChIP) to validate changes in epigenetic state at a targeted genomic region.

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Design of RNA-Based Translational Repressors,llent orthogonality, and logic capabilities. Riboregulators that provide the inverse function – turning off translation in response to a trigger RNA – are also versatile tools for sensing and efficiently implementing logic gates such as NAND or NOR. Toehold and three-way junction (3WJ) repressors ar

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Design and Assembly of Multilevel Transcriptional and Translational Regulators for Stringent Controy to stringently control gene expression due to the use of a single type of regulator that can be susceptible to unavoidable biomolecular fluctuations. In contrast, multilevel controllers (MLCs) employ several forms of regulation simultaneously to overcome this issue, ensuring a reduced basal expres

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