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Titlebook: Reporter Genes; A Practical Guide Donald S. Anson Book 2007 Humana Press 2007 Gene activity.Mammalian systems.Marker genes.Reporter gene ex

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Green Fluorescent Protein as a Tracer in Chimeric Tissues,whole sample as a consequence of form aldehyde (FA) fixation. We have therefore developed an alternative method to fix GFP in frozen tissue sections by FA vapor. This method prevents leakage and redistribution of GFP and allows any cytochemical method to be applied to unfixed adjacent serial sections.
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weil es nicht damit getan war, die Zugtiere durch eine Wärmekraftmaschine zu ersetzen. Das Rad diente beim Pferdefuhrwerk nicht zum Antrieb. Das war beim Antrieb durch eine Wärmekraftmaschine anders, die Antriebskraft mußte von den Rädern übertragen werden. Beim Pferdefuhrwerk wurde die Zugkraft vo
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,Methodologies for Staining and Visualisation of β-Galactosidase in Mouse Embryos and Tissues,taining with horseradish peroxidase and use as a tool for genotyping. Using these protocols, localization of β-galactosidase can be visualized throughout development and in adult tissues. β-Galactosidase staining may be used purely as a marker of gene expression and also as a tracer in cell lineage studies.
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Detection of GFP During Nervous System Development in ,,development on a cellular and molecular level. In the following chapter we describe how to prepare . embryos or larvae for live imaging or immunohistochemical staining and provide some guidelines for optimal GFP detection.
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