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Titlebook: Reporter Genes; A Practical Guide Donald S. Anson Book 2007 Humana Press 2007 Gene activity.Mammalian systems.Marker genes.Reporter gene ex

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书目名称Reporter Genes
副标题A Practical Guide
编辑Donald S. Anson
视频video
概述Provides practical guides for the use of the reporter genes most commonly used in mammalian systems including b-galactosidase, GFP and luciferase.Easy to follow protocols for the use of reporter genes
丛书名称Methods in Molecular Biology
图书封面Titlebook: Reporter Genes; A Practical Guide Donald S. Anson Book 2007 Humana Press 2007 Gene activity.Mammalian systems.Marker genes.Reporter gene ex
描述.Reporter genes have played, and continue to play, a vital role in many areas of biological research by providing a ready means for qualitative and quantitative assessment of the activity of genes and location of gene products in different environments. This book describes practical protocols for experimentation with the most useful reporter genes for mammalian systems that are available and will concentrate on those marker genes that are currently most commonly used. .
出版日期Book 2007
关键词Gene activity; Mammalian systems; Marker genes; Reporter gene expression; cell biology; gene expression; g
版次1
doihttps://doi.org/10.1007/978-1-59745-549-7
isbn_softcover978-1-62703-862-1
isbn_ebook978-1-59745-549-7Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 2007
The information of publication is updating

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Three-Dimensional Analysis of Molecular Signals with Episcopic Imaging Techniques,t and are readily converted to volume data sets..The two methods differ in how tissue architecture is visualized and hence how specific molecular signals are detected. EFIC uses endogenous, broad-range, tissue autofluorescence to reveal specimen structure. Addition of dyes to the wax embedding mediu
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Subcellular Imaging of Cancer Cells in Live Mice,nuclei increased their length 1.6 times. Cancer cells in capillaries over 8 μm in diameter were shown to migrate at up to 48.3 μm/h. With the use of dual-color fluorescent cells and the Olympus OV100, a highly sensitive whole-mouse imaging system with both macrooptics and microoptics, it is possible
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Noninvasive Imaging of Molecular Events with Bioluminescent Reporter Genes in Living Subjects,ly luciferase (or beetle luciferase) and renilla luciferase (isolated from sea pansy). Although both these luciferases can produce light following interaction with the substrates, structurally and biochemically they are very different. Here we describe the methods and applications of firefly and ren
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Detection of Reporter Gene Expression in Murine Airways, lung spaces. For GFP fluorescence, it is important to inflate the lungs with fixative prior to freezing and subsequent sectioning. For processing of nasal tissues for β-galactosidase expression, we expand on a protocol used in previously reported gene transfer studies.
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,Methodologies for Staining and Visualisation of β-Galactosidase in Mouse Embryos and Tissues,taining with horseradish peroxidase and use as a tool for genotyping. Using these protocols, localization of β-galactosidase can be visualized throughout development and in adult tissues. β-Galactosidase staining may be used purely as a marker of gene expression and also as a tracer in cell lineage
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