Titlebook: Reporter Gene Assays; Methods and Protocol Robert Damoiseaux,Samuel Hasson Book 2018 Springer Science+Business Media, LLC, part of Springer

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High-Content Reporter Assays,, fluorescent reporters do not. They suffer from artifacts such as compound fluorescence and cellular debris which makes the assessment of whole well fluorescence signals difficult. However, the use of high-content screening allows for the isolation of individual cells, segmentation and thus enables

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Data Mining and Computational Modeling of High-Throughput Screening Datasets,y datasets entering public and open databases such as ChEMBL and PubChem. The growth of academic HTS screening centers and the increasing move to academia for early stage drug discovery suggests a great need for the informatics tools and methods to mine such data and learn from it. Collaborative Dru

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Intravital Imaging of Human Melanoma Cells in the Mouse Ear Skin by Two-Photon Excitation Microscoptidimensional imaging down to single-cell detection sensitivity in vivo in deep tissues. Here we used 2PE microscopy to visualize green fluorescent protein (GFP) as a reporter gene in human melanoma cells implanted into the dermis of the mouse ear skin. We first provide a step-by-step methodology to

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Patricia Dranchak,John J. Moran,Ryan MacArthur,Camila Lopez-Anido,James Inglese,John Svaren

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Nathan Y. Bentolila,Raymond L. Barnhill,Claire Lugassy,Laurent A. Bentolila

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Ellen Siebring-van Olst,Victor W. van Beusechem Musiktheater besteht insofern eine Zweiteilung zwischen den „mittelbaren“ Interpreten, die als Führungskräfte die künstlerische Konzeption für die jeweilige Produktion entwickeln, und den „unmittelbaren“ Interpreten, die als geführte künstlerische Mitwirkende diese Konzeption umsetzen (Dechant 1985

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