Titlebook: Reporter Gene Assays; Methods and Protocol Robert Damoiseaux,Samuel Hasson Book 2018 Springer Science+Business Media, LLC, part of Springer

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Developing Mammalian Cellular Clock Models Using Firefly Luciferase Reporter,tissue types share a common molecular mechanism at the cellular and molecular level which underlies the rhythmic expression of genes. Individual cells are the functional units for rhythm generation and cell-based clock models offer experimental tractability for discovery. Cellular clock models can b

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High-Throughput Screening of a Luciferase Reporter of Gene Silencing on the Inactive X Chromosome, to a screening strategy for identifying factors that reactivate epigenetically silenced genes. This epigenetic luciferase reporter is subject to endogenous gene silencing mechanisms on the inactive X chromosome (Xi) in primary mouse cells and thus captures the multilayered nature of chromatin silen

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Making It All Work: Functional Genomics and Reporter Gene Assays,aling cascades, find new drug targets, or decipher the function of gene expression. The genome-wide scale of these experiments necessitates a different approach toward science than traditional single hypothesis driven research. High-throughput experimentation requires large project teams, automation

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Reporter Gene Assays Using Transfectable Functional Genomics Libraries,ies offer insight into fundamental cellular processes governing health and disease and can be utilized in an arrayed fashion which makes them uniquely suited to deconvolute complicated disease phenotypes and dissect biological networks that would otherwise be inaccessible. Here we give an overview o

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Reporter Gene Assays Using Viral Functional Genomics Libraries,ect cell lines and primary cells. One solution to this problem is the use of virus to introduce genomic reagents. This strategy is more commonplace now than ever before with libraries covering cDNAs, shDNAs, miRNAs, and guide RNAs readily available. Maintenance and use of these libraries are more ch

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Using YFP as a Reporter of Gene Expression in the Green Alga ,,ith promoter-fusion constructs in . is a powerful method for connecting metal-responsive regulation with .-regulatory elements, but overcoming expression-level variability between transformants and optimizing experimental conditions can be laborious. Here, we provide detailed protocols for the high-

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