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Titlebook: Reporter Gene Assays; Methods and Protocol Robert Damoiseaux,Samuel Hasson Book 2018 Springer Science+Business Media, LLC, part of Springer

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书目名称Reporter Gene Assays
副标题Methods and Protocol
编辑Robert Damoiseaux,Samuel Hasson
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Reporter Gene Assays; Methods and Protocol Robert Damoiseaux,Samuel Hasson Book 2018 Springer Science+Business Media, LLC, part of Springer
描述This volume explores the applications of reporter gene technology and the methodologies needed for their effective implementation. The chapters in this book cover practical topics such as how to integrate reporter constructs into cellular models, viral delivery, splicing applications, in vivo imaging, and a guide to the use of multi-cistronic constructs.  Additionally, chapters also include detailed mechanistic uses of reporter genes in cellular pathways, and a look at project and data management of screening applications.  Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Cutting-edge and thorough, .Reporter Gene Assays: Methods and Protocols. is a valuable resource for anyone who is interestedin learning more about reporter genes..
出版日期Book 2018
关键词genes; gene expression patterns; cellular functions; NFkB activation in immune cells; high-throughput dr
版次1
doihttps://doi.org/10.1007/978-1-4939-7724-6
isbn_softcover978-1-4939-9268-3
isbn_ebook978-1-4939-7724-6Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2018
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Methods in Molecular Biologyhttp://image.papertrans.cn/r/image/827355.jpg
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High-Throughput Firefly Luciferase Reporter Assays, identify chemical compounds capable of interfering with cellular signaling. Here, we present methods to prepare affordable firefly luciferase assay reagents and procedures to use these reagents in reporter gene high-throughput screening with large batches of 96-well cell culture plates.
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Using the 2A Protein Coexpression System: Multicistronic 2A Vectors Expressing Gene(s) of Interest llowed by a simple example of a “how to” protocol to concatenate multiple genes of interest, together with a reporter gene, into a single gene linked . 2As for easy identification or selection of transduced cells.
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Gene Expression Analysis by Arylsulfatase Assays in the Green Alga ,,, we describe protocols for arylsulfatase activity assays using ., activity of which can be measured either quantitatively or qualitatively, and in low (individual sample) or high (96-well format) throughput.
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