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Titlebook: Renal Cancer; Methods and Protocol Jack H. Mydlo Book 2001 Humana Press 2001

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Murine Animal Modelo evaluate the efficacy and safety of different treatment modalities because many mice can be treated at the same time with easy handling. Among several experimental models, murine renal carcinoma (Renca), which arises spontaneously in Balb/c mice, is the most frequently used for the assessment of c
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Matrix Metalloproteinases and Their Inhibitorsollagenases. Their physiological and pathological significance is to modulate the extracellular matrix—e. g., in embryogenesis, in the ovarian cycles, or in inflammatory diseases such as rheumatoid arthritis or fibrosis of the liver or kidney (.,.).
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Telomerase Assay in Renal Cancer-splicing problem (.). Thus, telomeres progressively shorten with age in somatic cells in culture and in vivo. In contrast, cancer cells and malignant cell lines retain telomere length despite repeated mitosis (.). This is believed to be an essential component of immortalization for most cells.
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Fluorescence , Hybridization (FISH) to Metaphase and Interphase Chromosomesnt, yet costly, time-consuming, and technically difficult. Isotopic hybridization in situ was rapidly superseded by nonisotopic techniques—especially those utilizing fluorescently labeled probes (.–.). This chapter describes basic methodology for the accomplishment of metaphase and interphase fluorescence . hybridization (FISH).
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