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Titlebook: Renal Cancer; Methods and Protocol Jack H. Mydlo Book 2001 Humana Press 2001

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Comparative Genomic HybridizationNA (Test DNA) is hybridized together with a sex-matched normal DNA (Reference DNA) onto normal metaphase spreads. Test DNA and Reference DNA are labeled with fluorochrome-conjugated reagents of different color, thereby allowing the detection of genetic imbalance in the abnormal cells.
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Analyzing the , Gene by RT-PCR, Western Blotting, and Immunohistochemistryall exons that make up the 1.1-kb . cDNA. The coding region starts in exon 5 and stops in exon 9, producing a 16.8-kDa cytoplasmic protein. The . locus contains the hereditary renal cell carcinoma (RCC) t(.;.) translocation, and also encompasses the . common fragile region (for review, .). Numerous
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Matrix Metalloproteinases and Their Inhibitorsdependent on a zinc ion in the active center. MMPs are separated in three groups: gelatinases (type IV-collagenases), stromelysins, and interstitial collagenases. Their physiological and pathological significance is to modulate the extracellular matrix—e. g., in embryogenesis, in the ovarian cycles,
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