Titlebook: Receptor and Ion Channel Detection in the Brain; Methods and Protocol Rafael Luján,Francisco Ciruela Book 2016 Springer Science+Business Me

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Post-embedding Immunohistochemistry in the Localisation of Receptors and Ion Channelsnoperoxidase for light microscopy and post-embedding immunogold for electron microscopy and also discuss the limitations inherent to these approaches. We describe the main scope and protocols which have been successfully utilised at our research centres for the examination and analysis of neuronal s

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Book 2016choosing the best method for the target proteins under study. This work serves as a useful guide for young researchers and students in training as well as for neurologists and established scientists who wish to extend their repertoire of techniques.

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0893-2336 guide for young researchers and students in training as well as for neurologists and established scientists who wish to extend their repertoire of techniques.978-1-4939-7983-7978-1-4939-3064-7Series ISSN 0893-2336 Series E-ISSN 1940-6045

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Subsynaptic Membrane Fractionationlysis allows the required validation with markers of each synaptic compartment and enables a direct identification of the localization of any particular synaptic receptor. Although it does not allow the detailed mapping achieved by electron microscopy, the possibility of concentrating the samples allows a greater sensitivity.

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Single Nanoparticle Tracking of Surface Ion Channels and Receptors in Brain Cellsin living brain cells. In this chapter, we describe the procedure and experimental steps necessary to perform single nanoparticle tracking of membrane neurotransmitter receptors and ion channels in living brain cells.

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Localization of GFP-Tagged Proteins at the Electron Microscopetection, current protocols, advantages, and pitfalls, and we describe a method, used in our laboratory, which combines confocal fluorescence microscopy with transmission electron microscopy (TEM) for the study of cell cultures at the ultrastructural level.