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Titlebook: Receptor and Ion Channel Detection in the Brain; Methods and Protocol Rafael Luján,Francisco Ciruela Book 2016 Springer Science+Business Me

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0893-2336 results.Contains well-organized themes, key notes and expertReceptor and Ion Channel Detection in the Brain provides state-of-the-art and up-to-date methodological information on molecular, neuroanatomical and functional techniques that are currently used to study neurotransmitter receptors and ion
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Book 2016functional techniques that are currently used to study neurotransmitter receptors and ion channels in the brain. The chapters have been contributed by world-wide recognized neuroscientists who explain in an easy and detailed way well established and tested protocols embracing molecular, cellular, su
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Co-immunoprecipitation from Brain) in native conditions. In brief, this technique consists of immunocapturing a protein of interest from a tissue lysate using a specific antibody. Subsequently, the immunocomplex containing the antibody, the antigen of interest and the antigen-associated proteins is precipitated by means of a Protei
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Investigation of Neurotransmitter Receptors in Brain Slices Using Cell Surface Biotinylationma membrane proteins in brain slices allows their separation from those present in intracellular organelles. Membrane-impermeable, thiol-cleavable and amine-reactive biotinylation reagents (e.g. EZ-link sulfo-NHS-SS-biotin) form a stable covalent linkage with primary amino groups of surface-exposed
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