Titlebook: Receptor Signal Transduction Protocols; Gary B. Willars,R. A. John Challiss Book 20042nd edition Humana Press 2004

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Immunocytochemical Identification of G-Protein-Coupled Receptor Expression and Localization, expression and localization of proteins both in model systems and their physiological context. This chapter details the theory and practice of the technique as well as lists the materials required. A general protocol is proposed, which can be adapted to suit the needs of individual investigators us

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Identification of G-Protein-Coupled Receptor mRNA Expression by Northern Blotting and , Hybridizati. hybridization (ISH) and Northern blotting are powerful methods for the localization of mRNAs and the study of regulation of mRNA expression. ISH combines the power of precise cellular localization with the ability to perform semiquantitative analysis of the mRNA level, whereas Northern blotting ha

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Generation of Model Cell Lines Expressing Recombinant G-Protein-Coupled Receptors, recombinant systems. In this respect, transfected mammalian cell lines constitute the most frequently used model for investigating the pharmacological and biochemical properties of these receptors. Several protocols have been described (based on the use of calcium phosphate precipitation, DEAE dext

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Covalent Modification of G-Proteins by Affinity Labeling,nsmembrane receptors function as the guanine nucleotide exchange factors (GEFs). A research goal may be to determine which G-protein α-subunit is activated by the receptor under investigation. In a membrane preparation obtained from cells or tissues this can be achieved in a seemingly straightforwar

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