Titlebook: Receptor Signal Transduction Protocols; Gary B. Willars,R. A. John Challiss Book 20042nd edition Humana Press 2004

Invigorate

,Identification and Quantitation of G-Protein α-Subunits,ins) has led to the development of specific techniques that can be used to identify which of these polypeptide(s) is involved on receptor activation by ligand. In addition, these methods can be used to probe the specificity of the interaction and to yield information about the stoichiometries involv

inspiration

Analysis of Function of Receptor-G-Protein and Receptor-RGS Fusion Proteins,ese signaling systems. Here we describe some of the various techniques and methods used in detail. The process of fusing the two components, the receptor and the G-protein α-subunit, result in a construct that acts as an agonist stimulated GTPase enzyme whose expression level can be accurately deter

开始没有

Sigmoidoscopy

奴才

Grating

Ubiquitination of G-Protein-Coupled Receptors,R with an epitope-tagged ubiquitin construct in a heterologous expression system. Modification by ubiquitin of the GPCR resulting from agonist activation is detected by immunoprecipation and subsequent immunoblotting for the epitope-tagged ubiquitin. We use here the chemokine receptor CXCR4 as the m

障碍物

Receptor Mutagenesis Strategies for Examination of Structure-Function Relationships,ted mutagenesis using either the Altered Sites® II in vitro mutagenesis system or the GeneTailor® site-directed mutagenesis system can generate base substitutions/deletions/insertions that yield single/multiple amino acid substitutions/deletions/insertions and/or N- or C-terminal truncations in GPCR

legitimate

NUL

Charlatan