Titlebook: Real-Time C++; Efficient Object-Ori Christopher Kormanyos Book 20183rd edition Springer-Verlag GmbH Germany, part of Springer Nature 2018 C

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Christopher KormanyosBy definition, a fluorogenic enzyme substrate should not fluoresce at the wavelength used to monitor the assay; however, its enzymatic product should exhibit strong fluorescence at the appropriate monitoring wavelengths. The substrate-analyte ligand conjugate in FSIA serves as a modified enzyme subs

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Christopher Kormanyosght. Using fluorimetric microscopy, Rotman (1961) was able to detect single molecules of the enzyme β-galactosidase. Under more practical circumstances, the detection limits of fluorimetry are frequently limited by background fluorescence such as that arising from serum or other biological samples.

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ty and robust applicability, microbial transglutaminase (mTG), a protein-glutamine γ-glutamyltransferase isolated from ., emerged as a versatile tool for ADC manufacturing. Herein, we report a protocol for the site-specific, mTG-mediated modification of antibodies that allows the production of homog

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Christopher Kormanyosetail protocols for the expression and purification of trypsiligase as well as the synthesis of peptide (ester) substrates. In addition, we provide instructions for the bioconjugation reactions and for the qualitative and quantitative analysis of reaction progress and efficiency.

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Christopher Kormanyosrget tumor, and to be free of large aggregates which, even if soluble, are rapidly taken up by cells of the reticuloendothelial system. Therefore the older methods of antibody—enzyme coupling, such as periodate oxidation or glutaraldehyde activation (.) have been largely superseded by methods based

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Christopher Kormanyosapproaches to improving its performance. In this regard, merging and integrating other technologieswith widely known ELISAs have opened new avenues for the advancement of this immunoassay. Accordingly, the book provides cutting-edge information on integrated platforms such as ELISpot, plasmonic ELIS

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