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Titlebook: Rat Genomics; G. Thomas Hayman,Jennifer R. Smith,Mary Shimoyama Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 20

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楼主: Madison
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1064-3745 ideal combination of established experimental models, extensive physiological data, and genomic manipulability to facilitate exploration of the underlying biology..978-1-4939-9583-7978-1-4939-9581-3Series ISSN 1064-3745 Series E-ISSN 1940-6029
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The Rat: A Model Used in Biomedical Research,r gives an introduction to rat as a species and as a biomedical model, providing historical information, a brief introduction to the current state of rat research, and a perspective on the future of rat as a model for human disease.
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Embryonic Stem Cells and Gene Manipulation in Rat,st decade, not only foreign DNA introduction but also endogenous DNA modification has been successfully achieved with rat ESCs. Detailed protocols for establishment of bona fide rat ESCs and their use for production of gene-modified rats are described in this chapter.
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Library Preparation for Multiplexed Reduced Representation Bisulfite Sequencing with a Universal Addapter, which is expensive to generate, for each library. Here, we describe a library preparation method that utilizes a universal adapter and labels samples with unique indexed PCR primers, significantly reducing the cost of multiplexed RRBS.
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Mapping Mammary Tumor Traits in the Rat,tic modifiers of the nonmalignant tumor microenvironment that contribute to the formation, progression, and outcome of breast cancer. In this protocol review, we discuss the current methodologies for the three genetic mapping techniques in the rat that are widely used for identifying and testing the heritable genetic modifiers of breast cancer.
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