Titlebook: RNAi and microRNA-Mediated Gene Regulation in Stem Cells; Methods, Protocols, Baohong Zhang,Edmund J. Stellwag Book 2010 Springer Science+

认识

RNAi in Stem Cells: Current Status and Future Perspectives RNAi suppresses gene expression, examine some published RNAi approaches that have resulted in changes in stem cell function, and suggest the possible clinical relevance of this work in cancer therapy through targeting cancer stem cells.

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稀释前

功多汁水

Regulation and/or Repression of Cholinergic Differentiation of Murine Embryonic Stem Cells Using RNAfor suppressing cholinergic differentiation in murine embryonic stem cells by knockdown of expression of the transcription factor L3/Lhx8, a Lim homeobox gene family protein. This method will greatly facilitate functional analyses of the factors involved in neuronal differentiation and regeneration and contribute to cell transplantation studies.

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1064-3745 fy and analyze the expression and function of RNAi and miRNARecent stem cell research has revealed that miRNA and RNAi-mediated gene regulation is one of the vital determinates controlling the state of cell differentiation, with the small RNAs serving as key elements involved in regulatory network c

天赋

Establishing Efficient siRNA Knockdown in Stem Cells Using Fluorescent Oligonucleotidessive Western blot or quantitative real-time PCR (qRT-PCR) methods. Thus, the main culprit of ineffective knockdown, poor transfection, can be eliminated before engaging in tedious and time-consuming approaches for troubleshooting siRNA knockdown experiments.

宴会

Preparation and Analysis of MicroRNA Libraries Using the Illumina Massively Parallel Sequencing Techg studies. We describe a detailed procedure for the preparation of small RNA libraries for Illumina sequencing. We further comment on approaches for analyzing the resultant sequence data for measuring microRNA abundance.

格子架

A Recessive Genetic Screen for Components of the RNA Interference Pathway in Mouse Embryonic Stem Cee and select for RNAi mutants. Putative RNAi mutants were confirmed using a separate functional assay. The location of the gene trap was then identified using molecular techniques such as Splinkerette PCR. Our screening strategy successfully isolated several mutant clones of Argonaute2, a vital component of the RNAi pathway.

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