Titlebook: RNAi and microRNA-Mediated Gene Regulation in Stem Cells; Methods, Protocols, Baohong Zhang,Edmund J. Stellwag Book 2010 Springer Science+

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An Efficient Transfection Method for Mouse Embryonic Stem Cellsucing plasmids encoding the protein and (2) decreased SOX-2 and Oct-3/4 expression and subsequent morphological evidence of cell differentiation after introducing siRNA expression for suppressing SOX-2 and Oct-3/4, which are known to be essential for maintenance of stem cell properties in mouse ES c

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Book 2010 and mice, concluding with a section on recently developed methods for identification of miRNAs, including a general protocol for preparation and analysis of miRNA libraries for deep sequencing, knock down of a specific gene using miRNA-based shRNA, and miRNA expression analysis using qRT-PCR.Writte

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Baohong Zhang,Edmund J. StellwagIncludes methods for state-of-the-art deep sequencing, miRNA-based shRNA-induced gene knockdown and qRT-PCR technologies.Protocols to identify and analyze the expression and function of RNAi and miRNA

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978-1-4939-5749-1Springer Science+Business Media, LLC 2010

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https://doi.org/10.1007/978-1-60761-769-3Migration; PCR; Termination; Vivo; gene expression; genes; miRNAs; molecular biology; regulation; gene therap

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