| 书目名称 | RNAi and microRNA-Mediated Gene Regulation in Stem Cells | | 副标题 | Methods, Protocols, | | 编辑 | Baohong Zhang,Edmund J. Stellwag | | 视频video | | | 概述 | Includes methods for state-of-the-art deep sequencing, miRNA-based shRNA-induced gene knockdown and qRT-PCR technologies.Protocols to identify and analyze the expression and function of RNAi and miRNA | | 丛书名称 | Methods in Molecular Biology | | 图书封面 |  | | 描述 | Recent stem cell research has revealed that miRNA and RNAi-mediated gene regulation is one of the vital determinates controlling the state of cell differentiation, with the small RNAs serving as key elements involved in regulatory network control of pluripotent cell fate determination.In RNAi and microRNA-Mediated Gene Regulation in Stem Cells: Methods, Protocols, and Applications, expert authors from laboratories across the globe contribute an accessible compendium of up-to-date, proven methods focused on the study of the titular topic.Divided into three sections, the book first gives a brief introduction to RNAi and miRNAs in stem cells, with a focus on the current status of research and future perspectives, then it continues with detailed methods and protocols for RNAi screening, transfection, and the knockdown of specific genes and pathways in several animal species, including humans and mice, concluding with a section on recently developed methods for identification of miRNAs, including a general protocol for preparation and analysis of miRNA libraries for deep sequencing, knock down of a specific gene using miRNA-based shRNA, and miRNA expression analysis using qRT-PCR.Writte | | 出版日期 | Book 2010 | | 关键词 | Migration; PCR; Termination; Vivo; gene expression; genes; miRNAs; molecular biology; regulation; gene therap | | 版次 | 1 | | doi | https://doi.org/10.1007/978-1-60761-769-3 | | isbn_softcover | 978-1-4939-5749-1 | | isbn_ebook | 978-1-60761-769-3Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Springer Science+Business Media, LLC 2010 |
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发表于 2025-3-21 16:20:33
