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Titlebook: RNAi; Design and Applicati Sailen Barik Book 2008 Humana Press 2008 Evolution.Microarray.RNA interference.currentsara.genes.miRNA.siRNA

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Principles of Dicer Substrate (D-siRNA) Design and Functionhetic 21-mer siRNAs, suggesting a coupling of the Dicer-mediated processing step to the efficient assembly of the silencing complex, RISC. We describe the fundamental principles and experimental results leading to optimal Dicer substrates.
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Identifying siRNA-Induced Off-Targets by Microarray Analysisp of the human genome. To achieve this, small interfering RNAs (siRNAs), short synthetic duplexes having complete homology to the intended target, are introduced into cells to silence gene expression via a posttranscriptional cleavage mechanism. While siRNAs can be designed to effectively knock down
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Nasal Delivery of siRNAut chemical modifications can be applied intranasally. Various delivery vehicles have been tested and optimized. With a few exceptions, all promote significant uptake of siRNA into the lung tissue and offer protection against respiratory viruses such as respiratory syncytial virus (RSV), parainfluen
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RNA Interference as a Genetic Tool in Trypanosomese carried out ., making this a powerful means for the study of protein function . Several trypanosomatids, including those organisms responsible for human and animal diseases, naturally possess the machinery necessary for RNAi manipulations. This allows for the use of RNAi in unraveling many of the
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