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Titlebook: RNAi; Design and Applicati Sailen Barik Book 2008 Humana Press 2008 Evolution.Microarray.RNA interference.currentsara.genes.miRNA.siRNA

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书目名称RNAi
副标题Design and Applicati
编辑Sailen Barik
视频video
概述Step-by-step instructions in a laboratory bench protocol format.Detailed lists of the necessary equipment and reagents.Tips on troubleshooting from actual experience of the experts.Example data set in
丛书名称Methods in Molecular Biology
图书封面Titlebook: RNAi; Design and Applicati Sailen Barik Book 2008 Humana Press 2008 Evolution.Microarray.RNA interference.currentsara.genes.miRNA.siRNA
描述.RNA interference (RNAi), in which RNA silences RNA, is the most recent discovery to revolutionize the study of biology. In RNAi: Design and Applications, leaders in the field contribute state-of-the-art, easy to follow methods and bench protocols designed for practical, everyday use of RNAi in biological research. Divided into two parts, this comprehensive volume covers fundamentals including designs of RNAi, biochemical assay protocols for the major components of RNAi, and study of potential off-target effects, followed by an extensive section covering various applications of RNAi in diverse model organisms and systems, from antiviral and anticancer applications to altering flower color in plants. ..Cutting edge and clearly written, RNAi: Design and Applications enables a researcher with standard molecular biological training to perform major RNAi-related experiments and contribute to this revolutionary, growing field..
出版日期Book 2008
关键词Evolution; Microarray; RNA interference; currentsara; genes; miRNA; siRNA
版次1
doihttps://doi.org/10.1007/978-1-59745-191-8
isbn_softcover978-1-61737-819-5
isbn_ebook978-1-59745-191-8Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 2008
The information of publication is updating

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RNAi978-1-59745-191-8Series ISSN 1064-3745 Series E-ISSN 1940-6029
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Expression, Purification, and Analysis of Recombinant Drosophila Dicer-1 and Dicer-2 Enzymese III enzymes Dicer-1 and Dicer-2 generate miRNA and siRNA, respectively. We describe the methods for the expression, purification, and analysis of recombinant Dicer-1 and Dicer-2 enzymes. Our studies demonstrate that Dicer-1 and Dicer-2 display different substrate specificities and ATP requirements.
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