Titlebook: RNAi; Design and Applicati Sailen Barik Book 2008 Humana Press 2008 Evolution.Microarray.RNA interference.currentsara.genes.miRNA.siRNA

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Identification and Expression Analysis of Small RNAs During Developmentendogenous small RNAs, namely microRNAs (miRNAs), small interfering RNAs (siRNAs), and piwi-interacting RNAs (piRNAs/gsRNAs), have been characterized in vertebrates. The miRNAs are mainly involved in development and differentiation and alter gene expression through translational repression or mRNA c

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Screening and Identification of Virus-Encoded RNA Silencing Suppressorsthe course of evolution, the viruses have encoded proteins with the potential to suppress the host RNA silencing mechanism as a counterdefense strategy. The virus-encoded RNA silencing suppressors (RSSs) can serve as important biological tools to dissect the detailed RNA silencing pathways and also

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Temporal Control of Gene Silencing by , Electroporationthis, as the absence of a gene during the early stages of development will preclude its functional analysis during later stages. In contrast, RNAi technology allows one to achieve temporal control of gene silencing especially when used with oviparous animal models. In contrast to mammals, reptiles a

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Altering Flower Color in Transgenic Plants by RNAi-Mediated Engineering of Flavonoid Biosynthetic Panvolved in the pathway are well characterized such that it is possible to change flower color by engineering the pathway by overexpression of heterologous genes and/or suppression of endogenous genes in transgenic plants. Trimming an unnecessary pathway by suppression of endogenous genes is often es

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Book 2008 model organisms and systems, from antiviral and anticancer applications to altering flower color in plants. ..Cutting edge and clearly written, RNAi: Design and Applications enables a researcher with standard molecular biological training to perform major RNAi-related experiments and contribute to this revolutionary, growing field..