Titlebook: RNA-Protein Complexes and Interactions; Methods and Protocol Ren-Jang Lin Book 2016 Springer Science+Business Media New York 2016 RNA-bindi

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,Ribo-Proteomics Approach to Profile RNA–Protein and Protein–Protein Interaction Networks,many cases, these interactions are transient and highly dynamic. Therefore, capturing stable as well as transient interactions in living cells for the identification of protein-binding partners and the mapping of RNA-binding sequences is key to a successful establishment of the molecular interaction

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,Detection of Protein–Protein Interaction Within an RNA–Protein Complex Via Unnatural-Amino-Acid-Medction to remodel RNA–protein complexes. Precisely how the latter is achieved remains a mystery. We investigated this critical issue by using yeast Prp28p, an evolutionarily conserved DExD/H-box splicing factor, as a model system. To probe how Prp28p interacts with spliceosome, we strategically place

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Evolution of Cell-Type-Specific RNA Aptamers Via Live Cell-Based SELEX,d to a cell-surface antigen or a particular target cell population. In particular, it offers a facile selection strategy for some special cell-surface proteins that are original glycosylated or heavily post-translationally modified, and are unavailable in their native/active conformation after in vi

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Design of RNA-Binding Proteins: Manipulate Alternative Splicing in Human Cells with Artificial Spliing have been found to be closely associated with various human diseases; thus new approaches to modulate disease-associated splicing events will provide great therapeutic potentials. Here we report protocols for constructing novel artificial splicing factors that can be designed to specifically mod