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Titlebook: RNA-Protein Complexes and Interactions; Methods and Protocol Ren-Jang Lin Book 2016 Springer Science+Business Media New York 2016 RNA-bindi

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书目名称RNA-Protein Complexes and Interactions
副标题Methods and Protocol
编辑Ren-Jang Lin
视频video
概述Includes cutting-edge methods and protocols for studying RNA-protein interactions.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the
丛书名称Methods in Molecular Biology
图书封面Titlebook: RNA-Protein Complexes and Interactions; Methods and Protocol Ren-Jang Lin Book 2016 Springer Science+Business Media New York 2016 RNA-bindi
描述.This detailed volume explores the continuing techniques ofstudying RNA-protein complexes and interactions as research in these areasexpand.  After an introductory chapter,the book continues with ways to purify RNA-protein complexes assembled in cellsor in isolated cellular extracts, methods for measuring various biochemicalactivities of RNA-interacting proteins or ribonucleoproteins, biochemicalmethods for measuring direct RNA-protein contact, as well as various new orinnovative methods pertinent to the subject. Written for the highly successful .Methods in Molecular Biology. series,chapters contain brief introductions to their respective topics, lists of thenecessary materials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls..Authoritative and up-to-date, .RNA-ProteinComplexes and Interactions: Methods and Protocols. provides a set of usefulprotocols, both basic and advanced, designed to inspire researchers workingwith RNA and RNA-interacting proteins.
出版日期Book 2016
关键词RNA-binding proteins; RNA-protein complexes; Ribonucleoproteins; Direct RNA-protein contact; Bioinformat
版次1
doihttps://doi.org/10.1007/978-1-4939-3591-8
isbn_softcover978-1-4939-8087-1
isbn_ebook978-1-4939-3591-8Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2016
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Ren-Jang LinIncludes cutting-edge methods and protocols for studying RNA-protein interactions.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the
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Single-Turnover Kinetics of Methyl Transfer to tRNA by Methyltransferases,s can be directly assigned to the steps associated with the chemistry of methyl transfer, including the substrate binding affinity to the methyltransferase, the pre-chemistry re-arrangement of the active site, and the chemical step of methyl transfer. An additional advantage is that kinetic constant
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,Genome-Wide Profiling of RNA–Protein Interactions Using CLIP-Seq,ence genome and the identification of the crosslinking sites. Meanwhile, one or more amino acids of a crosslinked RBP can remain attached to its bound RNA due to incomplete digestion of the protein. As a result, reverse transcriptase may not read through the crosslink sites, and produce cDNA ending
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Informational Suppression to Probe RNA:RNA Interactions in the Context of Ribonucleoproteins: U1 anr U1” snRNAs with restored base-pairing to mutant 5′ss in different registers. Cells are transfected with combinations of minigenes and suppressor U1s, and the splicing patterns are analyzed by reverse transcription and semiquantitative PCR, followed by gel electrophoresis. The identity of suppresso
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