Titlebook: RNA-Based Technologies for Functional Genomics in Plants; Guiliang Tang,Sachin Teotia,Deepali Singh Book 2021 Springer Nature Switzerland

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Recent Advancements in MIGS Toward Gene Silencing Studies in Plants,of target transcripts. Several RNAi-based tools have been developed and optimized to study gene function and trait improvements in plants. One recent strategy based on miRNA-triggered secondary small interfering RNAs (siRNAs) through trans-acting siRNA (tasiRNA) pathway has been developed for effici

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Silencing and Expressing MicroRNAs in Plants Through Virus-Based Vectors,s. Expression vectors based on plant viruses have the advantages of easy infection, short time to get results, and high throughput nature. Here, we describe the approaches to discover microRNAs functions based on viral vectors in plants. We describe here the use of viral system as a tool for gene fu

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Suppressor to Survival: RNAi as a Molecular Weapon in Arms Race Between Virus and Host,lencing is an essential part of the cellular mechanism that controls transcription and plays an important role in defense against virus infection. Viruses evade the plant defense strategy, by encoding various “suppressor” molecules, which interact with the small RNA species or the components of host

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An Improved Virus-Induced Gene Silencing (VIGS) System in Zoysiagrass,ocedure of its application among dicot and monocot species varies based on types of vectors, plant susceptibility, as well as inoculation methods. Here, we described a simple yet efficient tobacco rattle virus (TRV)-based VIGS system for functional analysis of genes in zoysiagrass (. cv. “Zenith”),