Titlebook: RNA Spectroscopy; Methods and Protocol Véronique Arluison,Frank Wien Book 2020 Springer Science+Business Media, LLC, part of Springer Natur

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Encapsulation of Fluorescently Labeled RNAs into Surface-Tethered Vesicles for Single-Molecule FRETpsulation to data evaluation of single-molecule FRET experiments in TIRF microscopy. We present an optimized procedure that preserves RNA functionality and applies to investigations of, e.g., large ribozymes and RNAs, where direct immobilization is structurally not possible.

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Surface Plasmon Resonance for Investigating Molecular Interactions with RNA,The other one is an RNA-RNA complex formed between a shortened version of the TAR element of HIV-1 and a structured RNA, TAR. rationally designed to interact with TAR through loop-loop interactions. These methods can be easily adapted to other complexes involving RNA molecules and to other SPR instr

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Site-Specific Spin Labeling of RNA for NMR and EPR Structural Studies,tic Resonance (EPR) to study biological macromolecules. Combining NMR and EPR is emerging as a new approach to investigate the architecture of biological systems..Here, we describe an efficient protocol to introduce a paramagnetic probe into a RNA at a specific position. This method enables various

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. Equations and figurers quantify the phenomena being described and provide the reader with the tools to tradeoff various performance features. The discussions 978-3-031-00406-3978-3-031-01534-2Series ISSN 1932-6076 Series E-ISSN 1932-6084

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Reka A. Haraszti,Joerg E. Braun. Equations and figurers quantify the phenomena being described and provide the reader with the tools to tradeoff various performance features. The discussions 978-3-031-00406-3978-3-031-01534-2Series ISSN 1932-6076 Series E-ISSN 1932-6084

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Serena Bernacchi. Equations and figurers quantify the phenomena being described and provide the reader with the tools to tradeoff various performance features. The discussions 978-3-031-00406-3978-3-031-01534-2Series ISSN 1932-6076 Series E-ISSN 1932-6084

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Véronique Arluison,Frank WienIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts

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Methods in Molecular Biologyhttp://image.papertrans.cn/r/image/820194.jpg