Titlebook: RNA Spectroscopy; Methods and Protocol Véronique Arluison,Frank Wien Book 2020 Springer Science+Business Media, LLC, part of Springer Natur

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1064-3745 ation advice from the experts.This volume looks at the different spectroscopic and biophysical methods used by researchers to study the structure and folding of RNA, and to follow their interactions with proteins. The chapters in this book cover topics such as single-molecule spectroscopy of multipl

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Dynamic Light Scattering Analysis on RNA Associated to Proteins,d from the genome of HIV-1 in association with the viral precursor Pr55.. These interactions are crucial for the specific selection of the viral genomic RNA (gRNA) from the bulk of the viral spliced and cellular RNAs. This chapter displays how DLS allows to characterize the interactions that regulate the early steps of viral assembly.

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Native Electrospray Ionization Mass Spectrometry of RNA-Ligand Complexes,ovide a detailed protocol for native ESI-MS analysis of RNA complexes. As an example, we present the analysis of the HIV-1 genomic RNA dimerization initiation site (DIS) extended duplex dimer bound to the aminoglycoside antibiotic lividomycin.

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RNA Folding and Unfolding Under Force: Single-Molecule Experiments and Their Analysis,tate transitions are blurred in a noisy signal. In order to cope with this limitation, we have implemented a novel strategy to analyze the data, which uses a hidden Markov modeling procedure. A representative example of such an analysis is presented.