Titlebook: RNA Remodeling Proteins; Methods and Protocol Marc Boudvillain Book 2021Latest edition Springer Science+Business Media, LLC, part of Spring

其他

A Simple Fluorescence Microplate Assay to Monitor RNA-DNA Hybrid Unwinding by the Bacterial Transcrelectrophoresis. Here, we describe a fluorescence-based microplate assay that informs on Rho helicase activity in a matter of minutes and allows the multiplexed analysis of conditions required for primary biochemical characterization or for drug screening.

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Using Magnetic Tweezers to Unravel the Mechanism of the G-quadruplex Binding and Unwinding Activititing the DHX36-induced changes in the lifetime of the DNA G-quadruplex under tension. The principle of the measurement can be broadly extended to the studies of other DNA or RNA G-quadruplex helicases.

filicide

Probing Transcriptome-Wide RNA Structural Changes Dependent on the DEAD-box Helicase Dbp2,mes in vivo. In this chapter, we describe a protocol for identification of RNA structural changes that are dependent on a . DEAD-box helicase Dbp2. Experiments detailed here can be adapted to the study of other RNA helicases and identification of putative remodeling targets in vivo.

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PAEAN

Vulnerary

Known Inhibitors of RNA Helicases and Their Therapeutic Potential,ases, the different types of compounds explored, the proposed inhibitory mechanisms of the compounds on the proteins, and the therapeutic potential of these compounds in the treatment of various disorders.

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,High-Throughput Protein–Nucleic Acid Interaction Assay Based on Protein-Induced Fluorescence Enhancthroughput. These properties predispose mwPIFE to become a method of choice for routine applications that require multiple parallel measurements such as buffer optimization, competition experiments, or screening chemical libraries for binding modulators.

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