Titlebook: RNA Remodeling Proteins; Methods and Protocol Marc Boudvillain Book 2021Latest edition Springer Science+Business Media, LLC, part of Spring

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书目名称RNA Remodeling Proteins影响因子(影响力)




书目名称RNA Remodeling Proteins影响因子(影响力)学科排名




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书目名称RNA Remodeling Proteins年度引用学科排名




书目名称RNA Remodeling Proteins读者反馈




书目名称RNA Remodeling Proteins读者反馈学科排名

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1064-3745 ation advice from the experts.This volume. .provides the most current methods to study RNA remodeling proteins. Chapters detail methods, ranging from basic to complex, procedures to identify RNA remodeling proteins and their cofactors, physiological RNA targets and biological functions, and complex

RENAL

excrete

Happy Birthday: 30 Years of RNA Helicases,y the structure of RNA molecules and/or the functionality of ribonucleoprotein complexes. Ultimately, the action of RNA helicases results in changes in gene expression that allow the cell to perform crucial functions. In this chapter, we review established and emerging concepts for DEAD-box and DExH

LURE

合乎习俗

senile-dementia

Thermal Shift Assay for Characterizing the Stability of RNA Helicases and Their Interaction with Liof various ligands on that stability. It is particularly useful for the assaying of ligands that may stabilize oligomeric helicases, which rely on both substrates (oligonucleotides) and nucleotide cofactors (ATP analogues) for their stability in a functional state. In this chapter, we describe the r

crease

BRUNT

,High-Throughput Protein–Nucleic Acid Interaction Assay Based on Protein-Induced Fluorescence Enhanchese interactions is crucial for delineating the mechanisms governing transcription, genome duplication, and translation. Here we describe a detailed protocol for the quantitative analysis of protein–nucleic acid interactions based on protein-induced fluorescence enhancement (PIFE). While PIFE has m

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Probing RNA Helicase Conformational Changes by Single-Molecule FRET Microscopy,nd total internal reflection fluorescence (TIRF) microscopy to follow the conformational cycling of DEAD-box helicases on the single molecule level, using the eukaryotic translation initiation factor eIF4A as an illustrative example. Confocal microscopy enables the study of donor-acceptor-labeled mo