Titlebook: RNA Remodeling Proteins; Methods and Protocol Marc Boudvillain Book 2021Latest edition Springer Science+Business Media, LLC, part of Spring

Deceit

A Fluorescent Assay to Monitor Ligand-Dependent Closure of the Hexameric Rho Helicase Ring,ranscripts. During its catalytic cycle, Rho both loads onto and translocates along RNA through a series of tightly regulated, ligand-dependent conformational changes. Here we describe an assay to track Rho as it switches from an open-ring (RNA-loading) to a closed-ring (RNA-translocation) configurat

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LAPSE

Creatinine-Test

Using Magnetic Tweezers to Unravel the Mechanism of the G-quadruplex Binding and Unwinding Activitignetic tweezers-based single-molecule investigation of the binding of helicases to G-quadruplex structures and their ATP-dependent unwinding activity, using DHX36 (also known as RHAU and G4R1) helicase and a DNA G-quadruplex structure for an example. We specifically emphasize on the principle and me

insecticide

Characterization of the Brr2 RNA Helicase and Its Regulation by Other Spliceosomal Proteins Using Gg and unwinding assays. Here we describe detailed protocols for the production and purification of the spliceosomal Ski2-like RNA helicase, Brr2, and one of its regulatory factors, the Jab1 domain of the Prp8 protein from yeast. Furthermore, we include a production protocol for radioactively labeled

碎石

Monitoring Acetylation of the RNA Helicase DDX3X, a Protein Critical for Formation of Stress Granulhad previously shown that the lysine deacetylase HDAC6 is required for the formation of stress granules, but the substrate important for this function was not known. We recently found that the RNA helicase DDX3X is a novel HDAC6 substrate, which is critical for the formation of stress granules. Thro

BILE

Anthropoid

Deciphering the Dynamic Landscape of Transcription-Associated mRNP Quality Control Components Over ir nuclear retention and ultimately the degradation of their mRNA component by the 3′–5′ activity of the exosome-associated exonuclease Rrp6. This mRNP quality control process is stimulated by the NNS complex (Nrd1-Nab3-Sen1), which otherwise mediates termination, processing, and decay of ncRNAs. Th

慌张

conifer

Probing Transcriptome-Wide RNA Structural Changes Dependent on the DEAD-box Helicase Dbp2, proteins form the largest family in eukaryotes and have been shown to remodel RNA/RNP structures and clamping of RNA-binding proteins, both in vitro and in vivo. Nevertheless, for the majority of these enzymes, it is largely unclear what RNAs are targeted and where they modulate RNA/RNP structures